Fig. 1: ERα decreases BCa invasion.
From: ERα-mediated alterations in circ_0023642 and miR-490-5p signaling suppress bladder cancer invasion
a J82 cells were transduced with lentivirus carrying shERα to knock down ERα expression or shLuc as a control. UMUC3 cells were transduced with a lentivirus designed to overexpress ERα (oeERα) or with a vector control (Ctrl) and used in subsequent experiments. Western blot analyses were performed, and proteins were detected with an antibody against ERα. b Matrigel invasion assays were performed using J82 and UMUC3 cells transduced with the lentiviruses described above. c We used the TCGA database to analyze ERα expression in BCa samples, and the results revealed higher ERα expression in BCa tumors than in normal bladder tissues. d An analysis of the TCGA database revealed the expression of the ERα mRNA in patients with BCa presenting metastatic foci or patients without metastasis. Data shown in b and c are presented as means ± SD. *P < 0.05
