Fig. 5: mTOR interacts with and phosphorylates the STAT5.

Hepatic p-STAT5 was determined by western blots in L-TKO mice and control littermates (a), and in tumor and nontumor areas of L-TKO mice (b). c Liver tissues were lysed from rapamycin or vehicle treated 10-month-old L-TKO mice, and p-STAT5 was determined by western blots (n = 6). Western blots were quantified. ***P < 0.001. d HepG2 cells were immunoprecipitated with anti-mTOR antibody and the amounts of Raptor, and STAT5 co-purified with mTOR were determined by western blot. e HepG2 cells were immunoprecipitated with anti-STAT5 antibody and the amounts of mTOR and Raptor co-purified with STAT5 were determined by western blot. f Cultured hepatocytes cells from Ctrl and L-TKO mice were immunoprecipitated with anti-mTOR antibody and the precipitated mTOR was assayed for kinase activity against recombinant GST-tagged full-length STAT5