Fig. 6: Repression of MCL1 shows similar effect to THZ1 in combination with ABT-263. | Cell Death & Disease

Fig. 6: Repression of MCL1 shows similar effect to THZ1 in combination with ABT-263.

From: CDK7 inhibitor THZ1 inhibits MCL1 synthesis and drives cholangiocarcinoma apoptosis in combination with BCL2/BCL-XL inhibitor ABT-263

Fig. 6

a HuCCT1 and HuH28 cells were transfected with indicated siRNA for 48 h and then cultured in medium with or without ABT-263 at a concentration of 2 μM (HuCCT1) or 1 μM (HuH28) for another 48 h. Cell viability was measured by CCK-8 assay. Data represent mean ± SEM of three independent replicates (**P < 0.01; ***P < 0.001), b, c Cells were transfected with indicated siRNA for 48 h and then cultured in medium with or without ABT-263 at the above-mentioned concentrations for another 48 h. The apoptosis was detected by the Annexin-V/PI assay and Caspase 3/7 activity (**P < 0.01; ***P < 0.001). d Cells were treated with different concentrations of Triptolide for 24 h. MCL1 protein expression was analyzed by western blotting. e, f HuCCT1 and HuH28 cells were treated with Triptolide and ABT-263 at indicated concentrations for 48 h. Cell viability was measured by CCK-8 assay. Cell apoptosis was measured by Caspase 3/7 activity assay. Data represent mean ± SEM of three independent replicates. (**P < 0.01; ***P < 0.001)

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