Fig. 3: Autophagic flux in A549 and NCI-H1975 cells as well as in their transplanted tumors after vismodegib exposure. | Cell Death & Disease

Fig. 3: Autophagic flux in A549 and NCI-H1975 cells as well as in their transplanted tumors after vismodegib exposure.

From: Regulating autophagy facilitated therapeutic efficacy of the sonic Hedgehog pathway inhibition on lung adenocarcinoma through GLI2 suppression and ROS production

Fig. 3

a Autophagosomes and autolysosomes accumulation in A549 and NCI-H1975 cells after vismodegib treatment. Cell were treated with vismodegib for 48 h and prepared for TEM analysis. The specific autophagosomes vesicles were marked as a red arrow, while the autolysosomes were marked as a green arrow (N = nuclear). b Autophagosomes and autolysosomes accumulation in A549 and NCI-H1975 transplanted tumors. Mice were killed after a 28-day treatment of vismodegib, and the tumor tissues were resected for TEM sample preparation immediately. The specific autophagosomes vesicles were marked as a red arrow, while the autolysosomes were marked as a green arrow (N = nuclear). c, d Autophagic flux in A549 and NCI-H1975 cells after vismodegib treatment. The autophagosomes were marked by cyto-ID dye, while the lysosomes were stained by lysotracker DND99. All the experiments have been repeated for three times

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