Fig. 4: Enhanced cytotoxic effect of vismodegib in LUAD cells after autophagy blockage.

a, c Autophagy blockage by siRNAs against ATG5 and ATG7. Cells were transfected with ATG5 and ATG7 siRNAs for 48 h and then lysed for western blot analysis. Sicontrol is a sequence that anti-luciferase and is used as a negative control. b, d Enhanced cytotoxic effect of vismodegib in LUAD cells after ATG5 and ATG7 knockdown. The MTT assay has been repeated for three times