Fig. 4: MLN4924 enhances apoptosis and necroptosis induction by TNFR1 in myeloma cells. | Cell Death & Disease

Fig. 4: MLN4924 enhances apoptosis and necroptosis induction by TNFR1 in myeloma cells.

From: The NEDD8-activating enzyme inhibitor MLN4924 sensitizes a TNFR1+ subgroup of multiple myeloma cells for TNF-induced cell death

Fig. 4

a Cells were stimulated with the TNFR1-specific TNF mutant Fc-TNF(32W/86T) and the TNFR2-specific agonist TNC-scTNF(143N/145R) in the presence and absence of MLN4924 (20 µM). Next day, cells were analyzed for viability. b Effect of zVAD-fmk (50 µM) and nec-1 (90 µM) on TNF (100 ng/ml)/MLN4924 (20 µM)-induced cell death after overnight stimulation. c Cells were challenged with the indicated combinations of Killer-TRAIL, anti-FLAG mAb M2-oligomerized Flag-CD95L, TNF and 20 µM MLN4924 overnight and were analyzed for cellular viability. Data shown are technical triplicates and representative of at least two independent experiments

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