Fig. 8: TNF/MLN4924 induces apoptosis in primary myeloma cells of a subgroup of patients. | Cell Death & Disease

Fig. 8: TNF/MLN4924 induces apoptosis in primary myeloma cells of a subgroup of patients.

From: The NEDD8-activating enzyme inhibitor MLN4924 sensitizes a TNFR1+ subgroup of multiple myeloma cells for TNF-induced cell death

Fig. 8

a, b Freshly purified primary MM cells were incubated with either TNF (100 ng/ml), MLN4924 (20 µM), a combination thereof or 1:1000 DMSO (the solvent for MLN4924). After 1 day annexin V/propidium iodide (PI) staining was performed by flow cytometry. A representative annexin V/propidium iodide (PI) staining panel is shown for one sample (# 11). % cells in the double-negative quadrant are highlighted. a Annexin V/PI double-negative cells (lower left quadrant) were considered as the “live cell fraction”. Relative survival, as shown in b, was obtained by normalization of the annexin V/PI double-negative fraction of each treatment group to the corresponding live cell fraction observed in the DMSO-treated control group. Results were analyzed by ANOVA (one-way, Bonferroni comparison of all pairs of columns. ***p < 0.001; n.s. not significant. Please note, considering TNF + MLN4924 treated cells showing >50 and <50% viability as separate groups resulted in again in significant (***p < 0.00) killing for the “<50%” subgroup and no significant difference for the “>50% subgroup

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