Fig. 2: TGF-β1 induces miR-133a expression via both Smad3 and p38MAPK signaling pathways. | Cell Death & Disease

Fig. 2: TGF-β1 induces miR-133a expression via both Smad3 and p38MAPK signaling pathways.

From: Transforming growth factor (TGF)-β1-induced miR-133a inhibits myofibroblast differentiation and pulmonary fibrosis

Fig. 2

HFL cells were stimulated with 1 ng/mL of TGF-β1 for different periods of time (a, c) or with various concentrations of TGF-β1 for 48 h (b, d). Cells were then harvested and subjected to western blot analysis of α-SMA and CTGF (a, b) protein expression or quantitative RT-PCR analysis of miR-133a expression (c, d). HFL cells were pretreated with 10 µM of Smad3 inhibitor (SIS3), p38MAPK inhibitor (SB203580) or vehicle (DMSO) for 30 min, and then stimulated with 1 ng/mL of TGF-β1 for 48 h. Cells were harvested and subjected to western blot analysis of α-SMA and CTGF protein expression (e) or quantitative RT-PCR analysis of miR-133a expression (f). Data are presented as the mean ± SEM. *P < 0.05 (n = 3–4)

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