Fig. 3: Quantitative proteomics and KEGG pathway enrichment analysis.

a Schematic diagram of TGF-β1 and miR-133a mimic treatment. HFL cells were treated with 1 ng/mL of TGF-β1 for 48 h, and then transfected with miR-133a mimic or its control for an additional 48 h. b Volcano plot displays the overall proteins identified with a P-value < 0.05 and > 1.2-fold cutoff. In all, 227 proteins were down-regulated whereas 646 proteins were upregulated following miR-133a overexpression. c Overview of significantly upregulated or down-regulated proteins in three miR-133a overexpressed groups (133a-1, -2, -3) as compared to three control groups (CTL-1, -2, -3). The heatmap represents the log2 (abundances) of the differentially expressed genes in different samples. The color key from blue to red represents the log2(abundances) from low to high. d KEGG pathway analysis of down-regulated proteins. e KEGG pathway analysis of upregulated proteins. The vertical axis represents the pathway category and the horizontal axis represents the enrichment score [−log(P-value)] of the pathway. Significantly enriched KEGG pathways (P < 0.05) are presented. The data were analyzed by DAVID bioinformatics tools