Fig. 5: Overexpression of miR-133a alters the phenotype of TGF-β1-treated HFL cells. | Cell Death & Disease

Fig. 5: Overexpression of miR-133a alters the phenotype of TGF-β1-treated HFL cells.

From: Transforming growth factor (TGF)-β1-induced miR-133a inhibits myofibroblast differentiation and pulmonary fibrosis

Fig. 5

HFL cells were treated with 1 ng/mL of TGF-β1 for 48 h, and then transfected with miR-133a mimic or its control (CTL) for an additional 48 h. a Total RNAs were harvested and subjected to quantitative RT-PCR analysis of Col1a1 and ACTA2 mRNA expression. Data are mean ± SEM (n = 3) with ***P < 0.001. b HFL cell differentiation was assessed by immunofluorescence staining for α-SMA. Left: representative staining images of α-SMA–positive stress fibers (green) and DAPI (blue) showing nuclei under confocal laser scanning microscopy. Right: bright field image and DAPI staining of nuclei (blue) (scale bar = 30 μm). c Quantitative RT-PCR analysis of MMP-2 and MMP-9 mRNA expression. d Culture media were collected and run on 10% SDS-PAGE contains 1% collagen. Gels were incubated with MMP activity testing buffer for a gelatin zymography assay and then stained with Coomassie blue (left). Total proteins harvested from whole cell lysis run on 10% SDS were used as internal controls (right)

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