Fig. 1: TM4SF5 expression induced alternative splicing variant CD44v8-10 depending on ZEB2 and ESRPs.

a–c Different lung epithelial cells transduced without or with HA-TM4SF5 retrovirus were processed for RT-PCR (a, b) or western blot analysis (c). d–g Diverse lung epithelial cells transduced with shRNA for a control (NS) or TM4SF5 sequences (shTM4SF5, #2 or #4, Table 1), or transduced with control (−) or HA-TM4SF5 plasmid-containing retrovirus ( + ) were processed for RT-PCR (d, f, and g) or western blot analysis (e). h–j Lung cells transduced with control (−) or FLAG-ESRP1 (h), HA-TM4SF5 retrovirus, siRNA (−) or siESRP1 (#1 or #2 sequence, Table 1) (i), and control plasmid- (−) or ZEB2 plasmid-containing retrovirus were processed for western blot analysis (h, i) or RT-PCR (j). k NCI-H727 cells were transduced with control (NS) or shTM4SF5-containing lentivirus (#4 sequence, Table 1) prior to qRT-PCR analysis. The p values were calculated by two-tailed unpaired Student’s t-test. P values <0.05 were considered statistically significant. l Analysis of TM4SF5-positive lung cell lines from the Cancer Cell Line Encyclopedia (CCLE) for expression levels of the indicated (blue boxed) molecules. Data shown represent three independent experiments. See also Figure S1