Fig. 2: Autophagic flux is reduced following pharmacological inhibition of early-stage autophagy. | Cell Death & Disease

Fig. 2: Autophagic flux is reduced following pharmacological inhibition of early-stage autophagy.

From: Effect of early-stage autophagy inhibition in BRAFV600E autophagy-dependent brain tumor cells

Fig. 2: Autophagic flux is reduced following pharmacological inhibition of early-stage autophagy.

a, b Representative histograms and quantifications (Dunnett’s multiple comparisons; mean ± s.e.m., n = 3. *p < 0.05) of basal and induced autophagy in parental and resistant MAF794 and AM38 cells. mCh-GFP-LC3 cells were exposed to standard and serum starvation media (EBSS) for 24 h in the presence or absence of SBI (a) or VPS34-IN1 (b) and analyzed by flow cytometry. Autophagic flux was determined by the ratio change in the median fluorescence intensity of mCherry:GFP and normalized to DMSO control

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