Fig. 5: Immunocytochemical analysis of NGN2-mediated neuronal conversion process.
From: Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair
a Lack of marker expression for neural progenitors during the reprogramming process. The expression in neural stem cells (NSCs) served as a positive control. b Time-course analysis of proliferating cells by staining with PCNA (n = 20 randomly selected fields from triplicate samples). c, d Incorporation of BrdU in NGN2-induced neuronal cells. Cells were treated with BrdU for the indicated duration after viral infection (b). OM, OEC culture medium; NM, neuronal induction medium. *P < 0.05, ***P < 0.001 by Student’s t-tests. Scale bar = 50 μm
