Fig. 7: RFGF21 induces relaxation of aorta via CaMKK2/ AMPKα mediated activation of eNOS. | Cell Death & Disease

Fig. 7: RFGF21 induces relaxation of aorta via CaMKK2/ AMPKα mediated activation of eNOS.

From: Fibroblast growth factor 21 Ameliorates diabetes-induced endothelial dysfunction in mouse aorta via activation of the CaMKK2/AMPKα signaling pathway

Fig. 7

a Dose-dependent relaxation of aorta from C57BL/6J mice to rFGF21 pretreated with FIIN-4 (10 μM) or Compound C (10 μM) for 30 mins (n = 7–12). b, c Phosphorylation level of eNOS (b) and AMPKα (c) in aortas from C57BL/6 J mice pretreated with FIIN-4 (10 μM) or Compound C (10 μM) for 30 mins and exposed to rFGF21 (0.01 mg/ml) for 3 mins as determined by western blot analysis (upper panel) and quantitation using ImageJ software (lower panel) (n = 3). d Dose-dependent relaxation of aorta from C57BL/6J mice to rFGF21 pretreated with STO-609 (5 μg/ml) for 30 mins (n = 9–10). e–g Phosphorylation level of eNOS (e), AMPKα (f) and ACC (g) in aortas from C57BL/6J mice pretreated with STO-609 (5 μg/ml) for 30 mins and exposed to rFGF21 (0.01 mg/ml) for 3 mins as determined by western blot analysis (upper panel) and quantitation using ImageJ software (lower panel) (n = 4). All data are presented as mean ± SEM. *p < 0.05 vs Control; #p < 0.05 vs FGF21

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