Fig. 3: Baclight assay to determine dead versus viable bacteria in the presence of neutrophils.
From: Degraded neutrophil extracellular traps promote the growth of Actinobacillus pleuropneumoniae
Live/dead bacterial viability staining of A.pp in the presence of absence of neutrophils was conducted (green = living cell/A.pp, red = dead cell/A.pp). Samples were incubated for 1 and 3 h as described in Fig. 1f. Per sample 8–12 pictures were taken and representative pictures from one experiment, time-point 3 h are shown (scale bar = 10 µm). Arrow head = living neutrophil, arrow = dead neutrophil, open arrow = NET structures, white *living A.pp, white #dead A.pp. As control for the neutrophils an untreated sample was included (PMN—A.pp)
