Fig. 6: NAD and adenosine as growth factor for A.pp and released from NETs.
From: Degraded neutrophil extracellular traps promote the growth of Actinobacillus pleuropneumoniae
a A.pp was grown for 3 h at 37 °C in the absence or presence of neutrophils (PMN) and DNase as described in Fig. 1e and f. A 5′-nucleotidase inhibitor was used to reduce adenosine production. The survival factor was calculated. Data shown are mean ± SD (n = 4, samples with and without inhibitor were analyzed with one-tailed paired Student’s t-test). b, c NAD was determined with ELISA and adenosine with a colorimetric assay in samples from NETs antimicrobial activity assay (Fig. 1e, f) but without A.pp. Data presented from n = 3 independent experiments. Data are presented with mean ± SD (n = 3, measured in one technical detection run, one-way ANOVA in each sample group, followed by Dunnett’s multiple comparison test to RPMI). d H. influenzae was grown for 3 h at 37 °C in the absence or presence of neutrophils (PMN) and DNase as described in Fig. 1 for A.pp. A 5′-nucleotidase inhibitor was used to reduce adenosine production. The survival factor was calculated and revealed that the inhibitor suppressed the growth benefit in the presence of DNase or degraded NETs. Data shown are mean ± SD (n = 3, ANOVA P = 0.0017, followed by Tukey’s multiple comparison test)
