Fig. 5: Transplantation of murine macrophages secreting IL-4 (M-IL-4) decreases infiltration of inflammatory cells and alveolar macrophage death.

Mice were exposed to LPS (15 mg/kg). After 0.5 h, PBS, M-IL-4 (2.5 × 105 cells/mouse), or M-Con (2.5 × 105 cells/mouse) was intratracheally transplanted (n = 6 for each group). The BALF was collected at 6 h after LPS administration, and the BAL cells were used to analyze by flow cytometry. a CD11b+ and Ly6C+ (monocyte) BAL cells identified by flow cytometry; b CD11b+ and Ly6G+ (granulocytes) BAL cells identified by flow cytometry; c CD11blow and CD11Chi (alveolar macrophage) BAL cells identified by flow cytometry; d Alveolar macrophage stained with 7AAD were measured by flow cytometry. *P < 0.05; **P < 0.01, the error bars represent ± SD