Fig. 5: CCR2 expression is transcriptionally regulated by β-catenin.

a The mRNA expression of β-catenin, CCR2 or Lef-1 in cells transfected with si-β-catenin, si-Lef-1 or β-catenin plasmids. b Immunoblot analysis of β-catenin and CCR2 in cells with inhibition of β-catenin and Lef-1, or β-catenin overexpression. c The CCR2 promoter luciferase construct (−2021/−311) CCR2 was cotransfected with pGL3-β-catenin, and promoter activities were detected in HCT116 and SW480 cells. d Selective deletion analyses identified both sites A and B were β-catenin-responsive regions in the CCR2 promoter. The schematic constructs are shown (left), and the bar graphs present the relative levels of luciferase activity (right). e A ChIP assay demonstrated the direct binding of β-catenin to TCF/LEF consensus-binding sites on the CCR2 promoter in CRC cells and tissues. Data represent the mean ± SD and are representative of three independent experiments. **P < 0.01, ***P < 0.001