Fig. 6: IMD 0354 suppresses osteoclastogenesis by mediating the phosphorylation of IKKβ and IkBα, inhibiting NF-kB nuclear translocation, and blocking the phosphorylation of ERK. | Cell Death & Disease

Fig. 6: IMD 0354 suppresses osteoclastogenesis by mediating the phosphorylation of IKKβ and IkBα, inhibiting NF-kB nuclear translocation, and blocking the phosphorylation of ERK.

From: The emerging role of IMD 0354 on bone homeostasis by suppressing osteoclastogenesis and bone resorption, but without affecting bone formation

Fig. 6: IMD 0354 suppresses osteoclastogenesis by mediating the phosphorylation of IKKβ and IkBα, inhibiting NF-kB nuclear translocation, and blocking the phosphorylation of ERK.The alternative text for this image may have been generated using AI.

a BMMs were pretreated with or without 1μM IMD 0354 for 2 h and then treated with 50 ng/mL RANKL for the indicated periods. Cell lysates were analyzed using western blotting. The expression of phosphorylated IKKα/β, IkBα, and P65 and total IKKα, IKKβ, IκBα, and P65 was assessed (n = 3). b The gray levels of phosphorylated IKKα/β, IκBα, and P65 were quantified and normalized to total IKKα, IKKβ, IκBα, and P65 using Image J (n = 3). c BMMs were pretreated with or without 1 μM IMD 0354 for 2 h and then treated with 50 ng/ml RANKL for the indicated periods. Cell lysates were analyzed using western blotting. The expression of phosphorylated ERK, P38, and JNK and total ERK, JNK, and P38 was examined (n = 3). d The gray levels of phosphorylated ERK, JNK, and P38 were quantified and normalized to total ERK, JNK, and P38 using Image J (n = 3). e Schematic representation of the experiments presented in this image. Values were normalized to GAPDH and negative control expression values. Data are presented as mean ± SD. *P < 0.05, ***P < 0.005, compared with the controls

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