Fig. 3: Knockdown Rac1 reversed increased cell invasion observed upon ΔNp63α knockdown.
From: ΔNp63α suppresses cells invasion by downregulating PKCγ/Rac1 signaling through miR-320a
A431 cells were transfected with NSC siRNA, p63 siRNA, and/or Rac1 siRNA as indicated. At 24 h after the second round of transfection, 8.0 × 104 cells were subjected to Matrigel-based invasion assay (a) and the number of invading cells was quantitated after 21 h (b). The y-axis indicates the average number of cells invaded per field. Error bars represent standard deviation. Significant changes (P ≤ 0.05) relative to NSC controls are indicated with an asterisk. c The change in protein expression was measured by immunoblot analysis using Rac1 and pRac1 (S71) antibodies to measure unphosphorylated and phosphorylated levels of Rac1, respectively. Immunoblot with β-actin was performed to confirm equivalent protein loading
