Fig. 4: Overexpression of a miR-320a mimic rescues the effect of ΔNp63α knockdown on Rac1 phosphorylation and cell invasion. | Cell Death & Disease

Fig. 4: Overexpression of a miR-320a mimic rescues the effect of ΔNp63α knockdown on Rac1 phosphorylation and cell invasion.

From: ΔNp63α suppresses cells invasion by downregulating PKCγ/Rac1 signaling through miR-320a

Fig. 4

A431 cells were transfected with either non-silencing control (NSC) or sip63 in conjunction with a negative control mimic or miR-320a mimic for two rounds of transfections. a The change in indicated protein levels were analyzed via immunoblotting with p63, Rac1, and pRac1 (S71) antibodies as indicated. Immunoblot with β-actin was performed to confirm equivalent protein loading. Twenty four hour after the second of transfection, 8.0 × 104 cells were subjected to Matrigel-based invasion assay (b) and the number of invading cells was quantitated after 21 h (c). The y-axis indicates the average number of cells invaded per field +1 standard deviation. Significant changes (P ≤ 0.05) relative to cells transfected with NSC and control mimic are indicated with an asterisk

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