Fig. 2: S1PR1 promote CRC cell proliferation, invasion, migration and liver metastasis in vitro and in vivo. | Cell Death & Disease

Fig. 2: S1PR1 promote CRC cell proliferation, invasion, migration and liver metastasis in vitro and in vivo.

From: The mechanism of the premetastatic niche facilitating colorectal cancer liver metastasis generated from myeloid-derived suppressor cells induced by the S1PR1–STAT3 signaling pathway

Fig. 2: S1PR1 promote CRC cell proliferation, invasion, migration and liver metastasis in vitro and in vivo.The alternative text for this image may have been generated using AI.

a S1PR1 protein expression levels in SW480 cells transduced with lenti-exS1PR1 and in HCT116 cells transduced with lenti-shS1PR1, as revealed using Western blotting. b S1PR1 overexpression significantly promoted the growth of SW480 and S1PR1 knockdown significantly inhibited the growth of HCT116, as revealed using an MTT assay. c A wound healing assay showed S1PR1 overexpression significantly promoted cell migration of SW480 and S1PR1 knockdown significantly inhibited the migration of HCT116, 36 h after wounding. d A transwell assay showed S1PR1 overexpression significantly promoted cell migration and invasion of SW480 and S1PR1 knockdown significantly inhibited the migration and invasion of HCT116. e The morphological characteristics of subcutaneous tumors with S1PR1 overexpression in SW480 and S1PR1 knockdown in HCT116. f Characteristic images of liver metastases after S1PR1 overexpression in SW480 and S1PR1 knockdown in HCT116. The data shown represent the mean ± s.e.m of a representative experiment performed in triplicate (*P < 0.05, **P < 0.01)

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