Fig. 4: Anti-proliferative effect of miR-4521 in MB cell lines.

MB cell lines were transfected with miR-4521 or miR-control. 24 h post-transfection cells were re-seeded (1 × 10⁴ cells/well). a Total cell number of the miR-4521 or miR-control cells is shown at different time points. b The relative viability of miRNA transfected cells was measured 3 days post transfection via a MTT assay. c A colony formation assay using miR-4521 transfected MB cells was performed (1 × 10³ cells/6-well). Representative pictures of the fixed and stained cells after 7 days growing. d The colonies of different cell lines and conditions were counted. All experiments were performed in triplicates. e DAOY and UW228.2 miR-control transfected cells after 0 and 24 h embedding. f DAOY and UW228.2 miR-4521 transfected cells after 0 and 24 h embedding. g The area of at least 10 spheroids was measured after embedding with Image J software. h The outgrowth rate of the miR-4521 transfected spheroids was calculated by comparing them to miR-control transfected spheroids after 24 h. Asterisks indicate significance (students t-test; *P < 0.05, ***P < 0.001), error bars indicate mean ± S.D