Fig. 2: VEGFA repression induces endometrial fibrosis in mice.

a The mRNA levels of VEGFA and VEGF164 in estrous uteri from Dox+ (N = 6) and Dox− mice (N = 6) were examined by qPCR. *P < 0.05, **P < 0.01. Unpaired t-test. b Uterine protein levels of VEGFA were significantly decreased in Dox− mice compared to Dox+ mice. N = 6, ***P < 0.001. Unpaired t-test. c Representative micrographs showing VEGFA, Ki67, active caspase 3, and collagen 1 levels by immunohistochemistry, e-cad (green), and α-SMA (red) levels by immunofluorescence, and Masson’s trichrome staining of murine uteri in estrus. Scale bar = 100 μm. N = 3, *P < 0.05. Unpaired t-test. d Hydroxyproline content was used to confirm uterine fibrosis. N = 3, **P < 0.01. Unpaired t-test