Fig. 3: SNRPB promotes metastasis of NSCLC cells in vitro.
From: SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26
Knockdown of SNRPB by shRNA vectors inhibited the migration and invasion of H1299 cells (a, b) and A549 cells (c, d). Left panels: representative images of migrating and invading A549 and H1299 cells with or without SNRPB knockdown. Right panels: quantification of the average number of migrating and invading cells/field in A549 and H1299 cells transduced with SNRPB shRNAs or control shRNA. e–f Ectopic expression of SNRPB promoted the migration and invasion of H460 cells as determined by transwell assays. Left panels: representative images of migrating and invading H460 cells transduced with vector control or SNRPB-Flag vectors. Right panels: quantification of the average number of migrating and invading cells/field in H460 cells. Proteins extracted from H1299 and A549 cell lines with SNRPB stably knockdown (g, h) or H460 cells that stably overexpressed SNRPB (i) were subjected to western blot to detect the expressions of EMT-related markers, such as Vimentin, MMP2, and MMP9. The expression of SNRPB and RAB26 was also measured
