Fig. 2: Effect of linc00645 on glioma cell proliferation, migration and invasion of glioma cells in vitro.

a, b qRT-PCR analysis indicated high level of linc00645 in glioblastoma tissues (n = 50) and glioma cell lines. c Kaplan-Meier algorithm showed high linc00645 was correlated with poor overall survival in 50 patients with GBM (log-rank test). d Linc00645 expression was efficiently knocked down by siRNAs in U251 and T98G cells as detected by qRT-PCR assays. e Ectopic expression of linc00645 in linc00645-transfected LN229 cells was determined by qRT-PCR. f Linc00645 expression levels in different subcellular fractions cells were measured by qRT-PCR in U251, T98G and LN229 cells. Black range indicates nuclear fraction, Gray range indicates cytoplasmic fraction. g MTT assay in si-NC or si-Linc 1# transfected U251 and T98G cells. (*P < 0.05, **P < 0.01, independent Student’s t test). h Colony formation assay in si-NC or si-Linc 1# transfected U251 and T98G cells. i Effect of linc00645 knockdown on U251 and T98G cell apoptosis. The apoptosis rate was displayed with bar graph. j Expression of Bcl-2 and Bax in si-NC or si-Linc 1# transfected cells as detected by western blot. The protein level was displayed with bar graph. k, l si-Linc 1# may inhibite migration and invasion ability in U251 and T98G cells. Data are shown as the mean ± SD of three independent experiments. (*P < 0.05, **P < 0.01)