Fig. 5: Linc00645 acted as a ceRNA by sponging miR-205-3p and regulated ZEB1 expression indirectly. | Cell Death & Disease

Fig. 5: Linc00645 acted as a ceRNA by sponging miR-205-3p and regulated ZEB1 expression indirectly.

From: RETRACTED ARTICLE: Long non-coding RNA linc00645 promotes TGF-β-induced epithelial–mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma

Fig. 5

a, b The relative luciferase activity was decreased after co-transfecting with miR-205-3p mimics and psiCHECK2-linc-WT in U251 and T98G cells. Luciferase activity was detected 24 h after transfection by using the dual luciferase assay. c–e Association between linc00645 and miR-205-3p with Ago2. f Real-time PCR was used to detect the linc00645 level change in the substrate of RIP assay in miR-205-3p-inhibitor transfecting glioma cells. RIP assay with Ago2 antibody was explored by using U251 or T98G cell extracts and RNA levels in immunoprecipitates were examined by qRT-PCR. RIP efficiency of Ago2 protein was detected by western blot. Data are shown as the mean ± SD of three independent experiments. (*P < 0.05, **P < 0.01)

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