Fig. 3: Ectopic expression of SPRY4 improves osteogenic differentiation of AIS MSCs in vitro. | Cell Death & Disease

Fig. 3: Ectopic expression of SPRY4 improves osteogenic differentiation of AIS MSCs in vitro.

From: SPRY4 is responsible for pathogenesis of adolescent idiopathic scoliosis by contributing to osteogenic differentiation and melatonin response of bone marrow-derived mesenchymal stem cells

Fig. 3

a, b AIS BM-MSCs from three patients were transduced with lentivirus overexpressing SPRY4 (lenti-SPRY4) or empty vector (lenti-NC). Efficiency of ectopic expression was verified by qRT-PCR (a) and western blot (b). c qRT-PCR analysis of osteogenic transcription factors and marker genes RUNX2, ALP, IBSP, OPN and COL1A1 on day 6 of osteogenic differentiation. d Western blot detected osteogenic transcription factors and marker genes IBSP, ALP, RUNX2 and OPN on day 6 of osteogenic differentiation. e, f ALP staining and relative ALP activity assays were performed on day 6 of osteogenic differentiation. g, h Calcium deposition by Alizarin red S staining and quantification were performed on day 12 of osteogenic differentiation. Data were from three independent experiments using BM-MSCs derived from three AIS patients. GAPDH was used as a loading control in both qRT-PCR and western blot analyses. Data are shown as the means ± SD

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