Fig. 2: OPA1 participates in regulation of mitochondrial network morphology in in vitro models of prion diseases. | Cell Death & Disease

Fig. 2: OPA1 participates in regulation of mitochondrial network morphology in in vitro models of prion diseases.

From: OPA1 overexpression ameliorates mitochondrial cristae remodeling, mitochondrial dysfunction, and neuronal apoptosis in prion diseases

Fig. 2

a Representative western blots and quantitative results of OPA1 protein levels. Control, OPA1 siRNA, and OPA1-overexpressed primary neurons before and after incubation with PrP106–126 (150 μM) for 12 h. b Primary neurons were transfected with OPA1 siRNA or OPA1 overexpression plasmid together with mito-GFP and then incubated with PrP106–126 (150 μM) for 12 h. Mitochondria morphology was observed by confocal microscopy. c The percentage of cells with fragmented mitochondria. d Mitochondria length. All in vitro experiments were repeated at least three times. Data are means ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001)

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