Fig. 6: OPA1 overexpression protects from PrP106–126-induced neuron apoptosis. | Cell Death & Disease

Fig. 6: OPA1 overexpression protects from PrP106–126-induced neuron apoptosis.

From: OPA1 overexpression ameliorates mitochondrial cristae remodeling, mitochondrial dysfunction, and neuronal apoptosis in prion diseases

Fig. 6

a Control and OPA1-overexpressed primary neurons were incubated with PrP106–126 (150 μM) for 24 h, and cell viability was measured by the CCK-8 Kit. b Immunoblots of mitochondrial cytochrome c proteins extracted from RML-infected C57 mouse brains, compared with the age-matched control. c Immunoblots of cytosol cytochrome c extracted from RML-infected C57 mouse brains, compared with the age-matched control. d Immunoblots of mitochondrial cytochrome c proteins of primary neurons. e Immunoblots of cytosol cytochrome c of primary neurons. f Western blots and quantitative results of cleaved caspase 3 levels in RML-infected C57 mouse brains compared with the age-matched control. g Western blots and quantitative results of cleaved caspase 3 levels in primary neurons. Ten prion-infected mice and wild-type mice were analyzed. All in vitro experiments were repeated at least three times. Data are means ± SEM. (**P < 0.01, ***P < 0.001)

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