Fig. 1: Affinity purification of Smad3 mRNA by miRIP in HepG2 cells.
From: microRNA-17 functions as an oncogene by downregulating Smad3 expression in hepatocellular carcinoma
a Smad3 mRNA and probe binding sites. Three segments (PCR1-3) denote the regions analyzed by qRT-PCR. b qRT-PCR analysis of region ‘PCR1’ of Smad3 mRNAs from P1 and P2 probe affinity purification in HepG2 cells, qRT-PCR analysis of region ‘PCR2’ of Smad3 mRNAs from P3 and P4 probe affinity purification in HepG2 cells, and qRT-PCR analysis of region ‘PCR3’ of Smad3 mRNAs from P5 probe affinity purification in HepG2 cells. c RT-PCR analysis of regions of Smad3 mRNAs from P5 probe and control probe affinity purification in HepG2 cells. d qRT-PCR of multiplex miRNAs data of P5 and control probe affinity purification in HepG2 cells. Shown data were normalized values for the enriched miRNAs (>2 folds). P1: probe 1; P2: probe 2; P3: probe 3; P4: probe 4; P5: probe 5; C: control probe
