Fig. 4: Downregulation of miR-17 inhibits the viability of HCC cells.
From: microRNA-17 functions as an oncogene by downregulating Smad3 expression in hepatocellular carcinoma
a Downregulation of miR-17 inhibits HCC cells proliferation. HepG2 and SMMC-7721 cells were transfected with negative control, miR-17 inhibitor or miR-17 inhibitor + Smad3 siRNA, and cells proliferation was measured by CCK-8 analysis at 24, 48, and 72 h after transfection (n = 6). b Cell-cycle distribution was analyzed by FACS analysis. HepG2 and SMMC-7721 cells were transfected with negative control, miR-17 inhibitor or miR-17 inhibitor + Smad3 siRNA (n = 3). c Analysis of the protein levels of p-Smad3, Smad3, and p21 expression in HepG2 and SMMC-7721 cells after transfection with negative control, miR-17 inhibitor or miR-17 inhibitor + Smad3 siRNA (n = 3). The data were subjected to Student’s t-test. Data were shown as mean ± SD, *p < 0.05, **p < 0.01. NC, negative control
