Fig. 5: Effect of miR-17 over-expression on tumorigenicity.
From: microRNA-17 functions as an oncogene by downregulating Smad3 expression in hepatocellular carcinoma
a Tumors after inoculation in Nod/Scid mice (4 weeks, n = 5). b The curve of tumor growth. Wilcoxon signed-ranks test was used for the comparison of tumor volumes versus NC transfectants. c The expression levels of miR-17 in miR-17 inhibitor transfected tumor cells group (right) and NC group (left) determined by RT-PCR (n = 5). d The protein level of p-Smad3, Smad3, and p21 increased in miR-17 inhibitor transfected tumor cells group (right) compared with NC group (left) (n = 5). e Ki-67 and PCNA staining in xenograft tumors. Ki-67 or PCNA positive cells decreased in miR-17 inhibitor transfected tumor cells group (right) compared with NC group (left) (n = 5). Data were shown as mean ± SD (n = 5), *p < 0.05
