Fig. 2: Impact of mevalonate pathway inhibitors and a γ-secretase inhibitor on Notch1 and Rab7.
From: Regulation of the Notch-ATM-abl axis by geranylgeranyl diphosphate synthase inhibition
a, b Western blots for Notch1 intracellular domain (NICD) and Rab7 after 72 h of treatment with various concentrations of test compounds in Jurkat (a) and Molt-4 cells (b). Tubulin is shown as a loading control. Blots are representative of three independent experiments. c Membrane, cytosolic, and whole-cell fractions of Rab7 protein. Western blots are representative of three independent experiments. d Impact of DGBP on NICD expression in other hematopoietic cell lines. Western blots are representative of three independent experiments. e, f Notch1 mRNA expression relative to 18S rRNA. Cells were treated with DGBP alone or a combination with geranylgeranyl diphosphate (GGPP) for 72 h. In all graphs, the bars represent means and standard deviations of three independent experiments (n = 3). Statistical significance was determined by two-way ANOVA with Tukey’s post hoc analysis. *p < 0.05 versus the corresponding control
