Fig. 4: ADP induces chemokine secretion in transgenic P2Y12+ U937 cells which can be inhibited with the specific P2Y12 antagonist PSB0739. | Cell Death & Disease

Fig. 4: ADP induces chemokine secretion in transgenic P2Y12+ U937 cells which can be inhibited with the specific P2Y12 antagonist PSB0739.

From: ADP secreted by dying melanoma cells mediates chemotaxis and chemokine secretion of macrophages via the purinergic receptor P2Y12

Fig. 4

a Gene expression analysis of transgenic U937 cells stimulated with 50 nM 2-MeSADP for 4 h. Heatmap depicts up-regulated cytokines, chemokines, and growth factors in 2-MeSADP-treated P2Y12+ U937 cells (P2Y12 ADP) compared to untreated P2Y12+ cells (P2Y12 CTRL), 2-MeSADP-treated EV cells (EV ADP), and untreated EV cells (EV CTRL) (n = 3). b Transgenic U937 cells were treated with 50 nM 2-MeSADP for 4 h and gene expression of the indicated chemokines was assessed by qRT-PCR. Gene expression was normalized to GAPDH (n = 4). c Chemokine concentrations in the cell supernatants of transgenic U937 cells were assessed by ELISA (n = 4). d P2Y12+ U937 cells were pre-treated with 10 µM PSB0739 prior to stimulation with 50 nM 2-MeSADP for 24 h. Chemokine concentrations in the cell supernatants were determined by ELISA (n = 4). Data is presented as mean ± SEM

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