Fig. 2: Apoptosis induced by matrine in ovarian cancer cells. | Cell Death & Disease

Fig. 2: Apoptosis induced by matrine in ovarian cancer cells.

From: Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways

Fig. 2

a Cells were measured by flow cytometry analysis of annexin V/PI double staining in A2780 and SKOV3 cells treated with various concentrations of matrine for 24 h. Region Q1 shows dead cells, Q2 shows late apoptotic cells, Q3 shows early apoptotic cells and Q4 shows living cells. Q2 and Q3 are collectively called apoptotic cells. b The distribution and percentage of living, apoptotic and dead cells for A2780 and SKOV3 cells were detected by flow cytometry. c A2780 and SKOV3 cells were treated with different concentrations of matrine for 24 h. Bcl-2 and Bax expression at protein level were evaluated by western blot. d The phosphorylation levels of PI3K and Akt in A2780 and SKOV3 cells were decreased after treatment of matrine. β-actin was used as an internal control

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