Fig. 3: Matrine induces the autophagy of ovarian cancer cells. | Cell Death & Disease

Fig. 3: Matrine induces the autophagy of ovarian cancer cells.

From: Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways

Fig. 3

a After exposure to matrine at 2.0 mg/mL, representative transmission electron microscopic images were obtained from matrine-treated cells as compared with control. The autophagosomes or autolysosome-like structures were indicated at the red arrows (scale bar: 2 μm). On the right panel were images with higher magnifications (scale bar: 0.5 μm). b Expressions of LC3-I/II and SQSTM1/p62 in matrine-treated A2780 and SKOV3 cells were determined by western blot. c A2780 and SKOV3 cells were transfected with GFP-LC3 plasmid for 24 h followed by exposure to matrine for 24 h. The representative images of GFP-LC3 puncta were viewed to monitor autophagosome formation by fluorescence microscopy. Each image is the representation of three independent experiments. Scale bar: 20 μm. d Western blot of the phosphorylation levels of Akt and mTOR in matrine-treated cells compared to the untreated cells. β-actin was used as a control

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