Fig. 4: NPI-0052 induced stabilization of the p53 family.

a, b Box plot representation of TP53 (a) and TP73 (b) expression levels (TPM: transcript per million). NC: normal cerebellum. **P < 0.001; ****P   < 0.00001. c, d Human MB cells DAOY (c) and ICb-1299 (d) were treated with 0.002 ng/μL NPI-0052 for 0, 6, 15 and 24 h. Cells were collected and a western blot of lysed cells probed with a primary anti-p73 (1/1000), anti-p53 (1/1000), GAPDH (1/5000) or vinculin (1/5000) antibodies. e, f DAOY cells were treated with two siRNA against p73 (sip73*1, sip73*2) for 48 h. e Cells were collected and a western blot of lysed cells for p73 and vinculin was performed. f Cell viability analysis in scramble, sip73*1 and sip73*2 DAOY cells after treatment with 0.002 or 0.01 ng/μL NPI-0052 for 24 h. **P  < 0.001; ***P  < 0.0001. g, h ICb-1299 cells were treated with a siRNA against p73 or p53 for 48 h. g Cells were collected and a RT-PCR for p73 and p53 was performed. ***P  < 0.0001 h Cell viability analysis in scramble, sip73 and sip53 ICb-1299 cells after treatment with 0.002 or 0.01 ng/μL NPI-0052 for 24 h. **P  < 0.001; ***P < 0.0001