Fig. 5: MTHFD2 and PAICS are related to neuroblastoma cell growth.
From: Combinatorial targeting of MTHFD2 and PAICS in purine synthesis as a novel therapeutic strategy
a, b Kaplan–Meier curves showing significant poorer prognosis in overall and event-free survival for patients with high expressions of MTHFD2 (a) and PAICS (b) using SEQC NB-498 dataset. The samples were straitified into high and low expressions based on the median value of the given gene expression. The differences in survival time between two groups were assessed by the log-rank test. c Western blot analyses revealing the SK-N-DZ stable knockdown cell lines of shRNA control, MTHFD2, PAICS, and MTHFD2/PAICS. d Cell viability of the SK-N-DZ stable knockdown cell lines was measured by MTS assays. e The cell viability in dual knockdown of MTHFD2 and PAICS (shMTHFD2/PAICS) has declined 48.4%, 50.2%, and 60.3% at 24, 48, and 72 h respectively. f–h The effects of SK-N-DZ stable knockdown cell lines on colony formation ability in representative plates (f) and quantitative analysis (g). h Single knockdown of MTHFD2 or PAICS alone was associated with approximately 32.1% and 29.6% declension, respectively, whereas dual knockdown of both MTHFD2 and PAICS resulted in a decrease of colony number to 46.5% compared with shLacZ cells
