Fig. 2: Chaetocin induces caspase-dependent apoptosis in GC cells. | Cell Death & Disease

Fig. 2: Chaetocin induces caspase-dependent apoptosis in GC cells.

From: ROS-mediated inactivation of the PI3K/AKT pathway is involved in the antigastric cancer effects of thioredoxin reductase-1 inhibitor chaetocin

Fig. 2: Chaetocin induces caspase-dependent apoptosis in GC cells.

a Apoptosis of HGC-27 and AGS cells was determined by flow cytometry following treatment with the indicated doses of chaetocin. Results are shown as mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, vs. control group. b Expression levels of cleaved-PARP, caspase-3, caspase-9 and caspase-8 in HGC-27 and AGS cells were analyzed by western blot. c Expression levels of BCL-2, BCL-XL, MCL-1, XIAP and survivin in HGC-27 and AGS cells were analyzed by western blot. HGC-27 and AGS cells were pretreated with 100 μM pan-caspase inhibitor z-VAD-fmk for 2 h and then cotreated with chaetocin (200 nM for HGC-27, 100 nM for AGS). d Expression levels of cleaved-PARP and caspase-3 were detected by western blot. e Cell viability was measured by CCK-8 assays. f Apoptosis was analyzed by flow cytometry. Results in (e) and (f) are shown as mean ± SD of three independent experiments. ***P < 0.001. All blots presented here are representative of three independent experiments

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