Fig. 3: MELAS ECs exhibit functional defects. | Cell Death & Disease

Fig. 3: MELAS ECs exhibit functional defects.

From: Mitochondrial 3243A > G mutation confers pro-atherogenic and pro-inflammatory properties in MELAS iPS derived endothelial cells

Fig. 3: MELAS ECs exhibit functional defects.The alternative text for this image may have been generated using AI.

a Scratch wound healing assays was performed on cMELAS and MELAS ECs. After 24 h, scratch area of MELAS ECs was observed to remained larger than the control, suggesting inefficient migration in MELAS ECs. Graphical representation illustrates the percentage of scratch area left after 24 h. b Tube formation assay was performed on cMELAS and MELAS ECs. Representative images demonstrate reduced tube formation in MELAS ECs. Quantitative analysis of several tube formation parameters showed overall poorer tube formation capacity of MELAS ECs. c Representative images of Ki67 immunostaining in cMELAS and MELAS ECs. Nuclei were stained in blue with DAPI. Scale bar = 100 μm. No distinct changes in percentage of Ki67 + cells between control and MELAS ECs (n > 100). d Flow analysis of Annexin-V showed higher percentage of MELAS ECs that were undergoing apoptosis. e Western blot and densitometric analysis shows higher levels of cleaved CASP7 protein expression in MELAS ECs as compared to cMELAS ECs. Expression of cleaved CASP7 was quantified and normalised to loading control β-ACTIN. f Representative images of cMELAS and MELAS ECs stained with VeCAD. Nuclei were stained in blue with DAPI. Scale bar = 100 μm. Quantification of mean fluorescence intensity illustrates MELAS ECs expressed lower levels of VeCAD protein. Error bars show SD of the mean. *p < 0.05, ***p < 0.001

Back to article page