Fig. 3: Visualization of cargo transfer mediated by tunneling nanotubes (TNTs) formed between stromal cells. | Cell Death & Disease

Fig. 3: Visualization of cargo transfer mediated by tunneling nanotubes (TNTs) formed between stromal cells.

From: Tunneling nanotube-mediated intercellular vesicle and protein transfer in the stroma-provided imatinib resistance in chronic myeloid leukemia cells

Fig. 3

a Frames from a time-lapse experiment that present the transport of two bulges along a TNT. Scale bar = 4 μm. b Velocity of cargo transfer in the time-lapse experiments (n = 18 events). c Representative images that show the presence of molecular motors inside TNTs. Green indicates the actin cytoskeleton. Blue indicates nuclei. Red indicates antibody-stained myosin Va (left), myosin VI (middle), and myosin VIIa (right). Scale bar = 5 μm. Single channels and a merged image are shown. d Representative images that show the presence of mitochondria inside TNTs (left panels). Green indicates actin. Blue indicates nuclei. Red indicates mitochondria that were stained with MitoTracker Deep Red. The 3D reconstruction shows in detail mitochondria that were present within the lumen of the TNT (right panels). Scale bar = 4 μm. e Representative image that shows the presence of cellular vesicles inside TNTs (left panels). Green indicates cell membranes that were stained with WGA-Alexa Fluor 488. Red indicates cytoplasmic vesicles that were stained with DiD. The 3D reconstruction shows in detail cytoplasmic vesicles that were present within the lumen of the TNT (right panels). Scale bar = 4 μm

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