Fig. 2: Expression of GFP from AAV vectors injected in the hippocampal DG (a–c) and AAV-mediated regulation of CXCR7 expression in vivo (d, e).

a AAV vectors (green) were successfully injected into the DG (white arrow), scale bar = 200 µm. b AAV-GFP-positive cells (green) were widely distributed in the DG, scale bar = 100 µm. c AAV-infected cells (green) in the GCL were colabeled with CXCR7 (red) and MAP2 (purple), as shown in the merged panels, indicating that the AAV vectors transfected CXCR7-positive GCs in the DG, upper panel scale bar = 100 µm, lower panel scale bar = 20 µm. d Representative images of the western blotting analysis of CXCR7 expression at weeks 4 and 9 after AAV injections. e Statistical analysis of CXCR7 expression in the hippocampus (n = 5 per group; week 4: shRNA compared with con-shRNA, p = 0.001; shRNA/CXCR7 compared with shRNA, p = 0.032; CXCR7 compared with con-CXCR7, p < 0.001; week 9: shRNA compared with con-shRNA, p < 0.001; shRNA/CXCR7 compared with shRNA, p = 0.026; CXCR7 compared with con-CXCR7, p < 0.001). One-way ANOVA with a post hoc Bonferroni test; *p < 0.05, **p < 0.01, and ***p < 0.001