Fig. 4: Ionizing radiation increases Tie2 expression and Tie2 signaling pathway activation in TDEC.

a Transcriptome analysis using Affymetrix® chip was performed on SRC3 GSC, and on GDC, TDEC and TDEC IR+ obtained from SRC3 GSC. Because there are no gene sets for ‘GSC’ or ‘GDC’ in databases like GO, KEGG, Reactome or MSigDB, this gene list was compiled by extensive literature search (Supplementary Table. S3) b The enrichment score of Tie2 signaling pathway members in TDEC IR− versus TDEC IR+ was determined from transcriptomic analysis (n = 3). The Tie2 signaling pathway members are listed in the “Materials and Methods” section. c Relative RNA expression of Tie2 in TDEC IR− and TDEC IR+ obtained from three different GSC (normalized to TDEC IR− obtained from each GSC). d–g Immunoblots of Tie2 d, P Tie2 e, P Akt and Akt f, and P ERK and ERK g in TDEC IR− and TDEC IR+ obtained from three different GSC. Blots are representative of at least 3 independent experiments. Blots were quantified and the graphs (bottom) show the protein expression ratio normalized to TDEC IR− obtained from each GSC