Fig. 3: Inhibition of autophagy decreased erastin-induced ferroptotic cell death. | Cell Death & Disease

Fig. 3: Inhibition of autophagy decreased erastin-induced ferroptotic cell death.

From: ROS-mediated autophagy increases intracellular iron levels and ferroptosis by ferritin and transferrin receptor regulation

Fig. 3

Wild type (n = Wi3) or autophagy-deficient cells (BECN1+/– (n = 3) or LC3B−/− (n = 3)) were treated with various doses of erastin for 24 h and autophagy-related proteins, ATG5, BECN1, and LC3B, were analyzed by western blot (a, b). *P < 0.05 indicates significant increase compared with vehicle. Wild type or autophagy-deficient cells (BECN1+/− (n = 3) or LC3B−/− (n = 3)) were pretreated with autophagy inhibitors (3-MA or CQ) 1 h before erastin (5 μM) treatment for 24 h, and autophagy-related proteins, ATG5, BECN1, and LC3B, were analyzed by western blot (c, d). *P < 0.05 indicates significant increase compared with vehicle. Cell viability was assayed 24 h after vehicle, erastin, or erastin plus autophagy inhibitor (3-MA or CQ) treatment in wild type (n = 12), BECN1+/− (n = 12), or LC3B−/− (n = 12) (e). All data shown are the mean ± SD from three independent experiments. *P < 0.05 indicates significant decrease compared with vehicle; †P < 0.05 indicates significant increase compared with erastin treatment

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