Fig. 4: Erastin-induced lipid peroxidation was disrupted in autophagy-deficient cells. | Cell Death & Disease

Fig. 4: Erastin-induced lipid peroxidation was disrupted in autophagy-deficient cells.

From: ROS-mediated autophagy increases intracellular iron levels and ferroptosis by ferritin and transferrin receptor regulation

Fig. 4

Wild type, BECN1+/−, or LC3B−/− fibroblastic cells were treated with vehicle or erastin (5 μM) for 24 h. Cytosolic ROS (a) and lipid peroxidation (b) were assayed for by flow cytometry using the fluorescent probes CellROX® Deep Red (cytosolic ROS) and C11-BODIPY (lipid peroxidation), respectively. Wild-type fibroblastic cells were treated with vehicle, erastin (5 μM), or erastin plus autophagy inhibitor (CQ or Baf A1) for 24 h and cytosolic ROS and lipid peroxidation were measured (c, d). Fluorescence of each probe was measured using FlowJo software program. The mean percentages ± SD of positive cells per total cells are shown in plots. Values are mean ± SD, n = 6. *P < 0.05 indicates significant increase compared with vehicle; NS, not significant

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