Fig. 3: Overexpressing or silencing PITPNA-AS1 could promote or inhibit the migration and EMT process of HCC cells. | Cell Death & Disease

Fig. 3: Overexpressing or silencing PITPNA-AS1 could promote or inhibit the migration and EMT process of HCC cells.

From: PITPNA-AS1 abrogates the inhibition of miR-876-5p on WNT5A to facilitate hepatocellular carcinoma progression

Fig. 3

a Transwell assay was conducted to measure the migratory ability. b Wound healing assay was carried out in HepG2 and Hep3B cells after indicated transfections. c Immunofluorescence (IF) was employed for the impact of PITPNA-AS1 upregulation or downregulation on the expression of EMT markers (E-cadherin and N-cadherin). d Western blot analysis of the levels of E-cadherin, N-cadherin, MMP2, and MMP9 in two transfected cells. *P < 0.05, **P < 0.01 indicated statistically significant differences. PITPNA-AS1 phosphatidylinositol transfer protein alpha antisense RNA 1, HCC hepatocellular carcinoma, IF immunofluorescence, EMT epithelial–mesenchymal transition, MMP2 matrix metalloprotein, MMP9 matrix metalloprotein 9

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