Fig. 1: The effects of ActD-induced nucleolar stress on embryo implantation.
From: Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines
a Delayed embryo implantation is activated by ActD at a dosage of 27.5 μg/mouse. b Immunofluorescence was used to indicate the location of NPM1 in the control and ActD-treated mouse uterus. Bar = 50 μm. c Western blot of the p53 protein in mouse uteri on days 8 and 9 (D8, mouse treated with vehicle or ActD for 1 day; D9, mouse treated with vehicle or ActD for 2 days). β-Actin was used as a loading control. d Real-time PCR analysis of the Its1, p21 and Mdm2 mRNA levels in mouse uteri on days 8 and 9. e The location of NPM1 in the luminal epithelial cells treated with ActD for 12 h. Bar = 25 μm. f Western blot of the p53 protein in ActD-treated luminal epithelial cells. Cytokeratin18 was used as a marker of luminal epithelium. Vimentin was used as a marker of stromal cells. β-Actin was used as a loading control. g Real-time PCR analysis of the Its1, p21 and Mdm2 mRNA levels in the ActD-treated luminal epithelial cells. Data are presented as the mean ± SD, *p < 0.05
