Fig. 5: Induction of nucleolar stress in uterine luminal epithelial cells.
From: Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines
a The location of NPM1 in the luminal epithelial cells treated with 100 ng/ml TNFα for 6 h. Bar = 25 μm. b The lactate concentration changes of medium after embryos cultured for 48 h. c The location of NPM1 in the luminal epithelial cells treated with 20 mM lactate for 2 h. Bar = 25 μm. d Western blot of the p53 and p-Stat3 proteins in the lactate-treated luminal epithelial cells. Tubulin was used as a loading control. e Real-time PCR analysis of the Lif mRNA level in the lactate-treated luminal epithelial cells. Data are presented as the mean ± SD, *p < 0.05
