Fig. 2: Intrathecal administration of miR-711 hairpin inhibitor increases Akt/GSK3α/β signaling and attenuates expression of PUMA and apoptotic marker fodrin cleavage at 24 h after SCI. | Cell Death & Disease

Fig. 2: Intrathecal administration of miR-711 hairpin inhibitor increases Akt/GSK3α/β signaling and attenuates expression of PUMA and apoptotic marker fodrin cleavage at 24 h after SCI.

From: Inhibition of microRNA-711 limits angiopoietin-1 and Akt changes, tissue damage, and motor dysfunction after contusive spinal cord injury in mice

Fig. 2: Intrathecal administration of miR-711 hairpin inhibitor increases Akt/GSK3α/β signaling and attenuates expression of PUMA and apoptotic marker fodrin cleavage at 24 h after SCI.

Mice were treated with miR-711 hairpin inhibitor or negative control (vehicle) at 5 min post-injury. a Representative western blots for total Akt, phosphorylated Akt (p-Akt, Ser473, Thr308), total and phosphorylated GSK3α/β, PUMA, cleaved α-fodrin. b–f Protein levels were quantified by densitometry, normalized to β-actin, and presented as fold change compared with sham-injured controls. All data are presented as independent data points. One-way ANOVA was performed followed by Newman-Keuls multiple comparisons test. n = 5/group. **p < 0.01, ***p < 0.001 vs. Sham; #p < 0.05, ##p < 0.01; ###p < 0.001 vs. SCI/Vehicle group

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